LOCUS       Exported                3937 bp ds-DNA    circular SYN 12-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0047_regulator_luxR(J23111)_corrected
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   iGEM ETC Zurich
  TITLE     Direct Submission
  JOURNAL   Exported 12 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      1..126
                     /note="T0"
     terminator      27..121
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     promoter        165..199
                     /note="J23111"
     RBS             208..219
                     /note="RBS (B0034)"
     CDS             226..981
                     /codon_start=1
                     /product="LuxR-Barcode"
                     /note="luxR (C0062)"
                     /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLAI
                     IYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAVNK
                     KSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPLIVP
                     SLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHLTNAQ
                     MKLNTTNRCQSISKAILTGAIDCPYFKN*"
     terminator      990..1118
                     /note="B0015"
                     /note="directional double term"
     terminator      1130..1216
                     /gene="Escherichia coli rrnB"
                     /note="T1"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1236..1262)
                     /note="AB185"
     misc_feature    1398..1589
                     /note="rop"
     misc_feature    1691..1831
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2002..2621)
                     /direction=LEFT
                     /note="pBR322_origin"
     primer_bind     complement(2612..2638)
                     /note="AB186"
     oriT            2641..2749
                     /note="incP origin of transfer"
     CDS             complement(2939..3799)
                     /note="AmpR"
     promoter        complement(3800..3891)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg
       61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      121 ccaagactag tcgccagggt tttcccagtc acgacgcggc cgcattgacg gctagctcag
      181 tcctaggtat agtgctagct actagagaaa gaggagaaat actagatgaa aaacataaat
      241 gccgacgaca catacagaat aattaataaa attaaagctt gtagaagcaa taatgatatt
      301 aatcaatgct tatctgatat gactaaaatg gtacattgtg aatattattt actcgcgatc
      361 atttatcctc attctatggt taaatctgat atttcaatcc tagataatta ccctaaaaaa
      421 tggaggcaat attatgatga cgctaattta ataaaatatg atcctatagt agattattct
      481 aactccaatc attcaccaat taattggaat atatttgaaa acaatgctgt aaataaaaaa
      541 tctccaaatg taattaaaga agcgaaaaca tcaggtctta tcactgggtt tagtttccct
      601 attcatacgg ctaacaatgg cttcggaatg cttagttttg cacattcaga aaaagacaac
      661 tatatagata gtttattttt acatgcgtgt atgaacatac cattaattgt tccttctcta
      721 gttgataatt atcgaaaaat aaatatagca aataataaat caaacaacga tttaaccaaa
      781 agagaaaaag aatgtttagc gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa
      841 atattaggtt gcagtgagcg tactgtcact ttccatttaa ccaatgcgca aatgaaactc
      901 aatacaacaa accgctgcca aagtatttct aaagcaattt taacaggagc aattgattgc
      961 ccatacttta aaaattaata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1021 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1081 cactggctca ccttcgggtg ggcctttctg cgtttataat taaaggcatc aaataaaacg
     1141 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1201 cctgagtagg acaaatccgc cgccctagac agctgggcgc gcccccgccg catccatacc
     1261 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
     1321 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaattccccc
     1381 ttacacggag gcatcaagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     1441 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
     1501 cagacatctg tgaatcgctg cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
     1561 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1621 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     1681 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     1741 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     1801 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     1861 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1921 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1981 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2041 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2101 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2161 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2221 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2281 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2341 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2401 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     2461 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2521 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2581 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggccggccta
     2641 cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt
     2701 gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg
     2761 ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa
     2821 aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga
     2881 aaaggacaag ggtcgtccaa aaaaaaaggc tccaaaagga gcctttaatt gtatcggttt
     2941 accaatgttt aatcaggctc gcgccaattt ccgcaatctg acggttacgt tcatccatcg
     3001 tcgcctggct gccggtggta taaatcacca caatacggct cggtttacca tccgggccca
     3061 gcgccgcaat aatgccacgg ctgccacgtt cgcccgcacc gcttttatcc gcaataaacc
     3121 agccagccgg cagcgcgcta cgcagcagcg gacccgccac tttatccgct tccatccaat
     3181 caatcagttg ctggcggctt gccagggtca gcagctcacc cgtcagcagt ttacgcaggg
     3241 tggtcgccat tgctgccggc atggtggtat cacgttcatc gttcggaatc gcttcgttca
     3301 gttccggttc ccaacgatcc agacgggtca catgatcgcc catgttatgc agaaacgcgg
     3361 tcagttcttt cggaccgcca atggtcgtca gcagcaggtt cgccgcggtg ttatcgctca
     3421 tggtaatcgc cgcgctgcac agttcacgca cggtcatgcc atcggtcaga tgtttttcgg
     3481 tcaccgggct atattccacc agatcgttct ggctataatg aatacgacgg cccagttgtt
     3541 cctggcccgc atccacacgg ctcagaaccg caccgcacag cagcacttta aaggtgctca
     3601 tcatcggaaa acgttcttcc ggacgaaaag attccagaat tttgccgctg ttcagatcca
     3661 gttcaatata gcccacgcgc gcacccagtt gatcttccgc atctttcact ttcaccaggg
     3721 tttccggatg cgcaaacacc ggcaggcaaa acgccgcaaa aaacggaatc agcgccacac
     3781 gaaaatgctg aatgctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggaat
     3901 taaaaaaaag cccgctcatt aggcgggcta ctaattt
//
